Two A<SUB>weak</SUB>B Cases with Aw10 Allele Separated by Allele-specific Sequencing

Two A_weakB Cases with Aw10 Allele Separated by Allele-specific Sequencing

대립유전자 특이 시퀀싱으로 분리한 Aw10 대립유전자를 가진 2예

The Korean Society of Blood Transfusion 2011;22:59−64

Published online April 30, 2011

Eun-Jeong Won, Duck Cho, Myung-Geun Shin, So-Yong Kwon¹, Nam-Sun Cho¹, Dong-Wook Ryang
원은정ㆍ조 덕ㆍ신명근ㆍ권소영¹ㆍ조남선¹ㆍ양동욱

Abstract: We separated an Aw10 allele by allele-specific sequencing in two Aw10/B101 samples that had the AweakB phenotype. Two samples with the A102/B101 genotype were also tested as a control. The reverse primers using position 930 at exon7 were designed for allele-specific sequencing. The differential positions were a total of 52 points for distinguishing the A-allele from the B-allele. Although overlaps with another haplotype allele that showed a minor chromatographic peak were observed in almost all the points, the specific allele-separation rate was 100% (52/52) by assessing the dominance in the chromatographic peak height. Based on the separation rate in the two cases with Aw10/B101 and the two AB controls, allele-specific sequencing is a convenient and reliable method for the separating the A-allele and B-allele in a clinical laboratory. (Korean J Blood Transfus 2011;22:59-64); Keywords : ABO gene, Allele separation, Allele-specific sequencing

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