Cord Blood Volume Reduction Using the Automated Devices SepaxⰒ S-100 and AXPTM AutoXpress Platform
SepaxⰒ S-100와 AXPTM AutoXpress Platform을 이용한 제대혈 혈장 및 적혈구 제거
Eun Youn Roh1,2, Sue Shin1,2, Jae Hee Lee2, Dong Ho Kim2, Jee Young Chang2, Jung Ja Hong2, Eun Young Song3, Jong Hyun Yoon1,2
노은연1,2ㆍ신수1,2ㆍ이재희2ㆍ김동호2ㆍ장지영2ㆍ홍정자2ㆍ송은영3ㆍ윤종현1,2
노은연1,2ㆍ신수1,2ㆍ이재희2ㆍ김동호2ㆍ장지영2ㆍ홍정자2ㆍ송은영3ㆍ윤종현1,2
- Abstract
- Background: A successful cord blood (CB) bank requires long-term storage of a large number of CB units with good quality. To provide an optimal storage condition and to solve issues of space, many techniques have been developed to remove the plasma and RBC with maintenance of the quality and cell dose. We compared the results of the use of two different automated systems with the hydroxyethyl starch (HES) sedimentation method used in routine manual processing.Methods: A total of 38 donated CB units with informed consent (18 June 2007∼16 July 2007) were randomly selected and enrolled. We performed volume reduction of 20 units with the SepaxⰒ S-100 (Sepax), 18 units with the AXPTM AutoXpress Platform (AXP), and compared the levels of nucleated cell recoveries, viability, and CD34+ cell/total nucleated cells (TNC) with the levels of 20 units processed using the HES method in the same period. The correlations of the initial TNC with cell recoveries were also evaluated.Results: The mean values of the TNC recoveries, viabilities, and CD34+ cell/TNC among the three methods were similar (P=0.75; P=0.13; P=0.75). However, the MNC recovery was significantly higher with the use of the AXP system (89.2%, P＜0.001) than the other methods. The Sepax system also showed a reduced SD for TNC recovery. Moreover, cell recovery rates were independent of the initial TNC counts. Conclusion: The Sepax and AXP automated cord blood process systems allow similar cell recoveries and viability as compared with the HES method. With the results of this study, a cord blood bank may be able to use an automated system considering the facility, personnel and the workload. (Korean J Blood Transfus 2007;18:219-226)
- Keywords : Cord blood, Volume reduction, Hydroxyethyl starch sedimentation, Automated system
- December 2024, 35 (3)
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