The Effect of Magnesium Sulfate on Platelets Preserved at Cold Temperature

혈소판제제의 냉장보존에 있어서 마그네슘의 유효성

The Korean Journal of Blood Transfusion 2007;18:188−193

Published online December 30, 2007

Yong Gon Cho¹, Jae Hyeon Lee, Jeong Tae Kim, Dal Sik Kim¹, Hye Soo Lee¹, Sam Im Choi¹
조용곤¹ㆍ이재현ㆍ김정태ㆍ김달식¹ㆍ이혜수¹ㆍ최삼임¹

Abstract: Background: It is known that magnesium can inhibit platelet function in vitro and ADP-induced platelet activation. We wished to demonstrate if magnesium could inhibit activation of platelets preserved at cold temperature. Methods: We incubated each mixture of platelets and various concentrations of magnesium sulfate (0∼10 mM) at room temperature and at a cold temperature (4^oC). On days 1, 3, 5, 7 and 9, we measured the platelet count, pH, LD level, glucose level, HCO₃^- level, lactate level, expression of CD62P, expression of annexin V, and ADP-induced aggregation of platelets.Results: The platelets stored at 4^oC showed a similar LD level but a lower platelet count, lactate level, and expression of CD62P and annexin V, and a higher pH and glucose level than platelets stored at room temperature. With an increasing magnesium concentration, expression of CD62P in the platelets stored at 4^oC was slightly decreased, but expression of annexin V was increased. ADP-induced aggregation of the platelets stored at 4^oC was not affected by magnesium.Conclusion: Magnesium did not effectively inhibit activation of platelets during preservation at cold temperature (4^oC). (Korean J Blood Transfus 2007;18:188-193); Keywords : Magnesium, Platelets, Preservation

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