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The Effect of Zoledronate on the Differentiation of Human Amniotic Fluid Derived Mesencymal Stem Cell into Adipocyte
Zoledronate가 인간 양수유래 중간엽 줄기세포로부터 지방세포로의 분화에 미치는 영향
The Korean Society of Blood Transfusion 2008;19:231−238
Published online December 31, 2008
© 2008 The Korean Society of Blood Transfusion.

Bo Sun Joo1, Joon Hyop An2, Byeong Min AnS1, Tae Young Choi1, Du Yeal Song3, Hyung Hoi Kim3, Hwa Sook Moon1,4
Background: Amniotic fluid is a rich source of fetal mesenchymal stem cells (MSCs). However, little is known about whether bisphosphonates affect the differentiation into adipocytes. Therefore, this study was aimed to investigate whether zoledronate influences the differentiation of AFMSCs into adipocytes. Methods: Amniotic fluid cells samples were obtained from 6 pregnant women by second trimester amniocentesis for performing fetal karyotyping. The cells were treated with various concentration (10−10, 10−8, 10−6 M) of zoledronate and the cells were analyzed over 21 days of culture. Differentiation into adipocytes was determined by oil-red O staining and for fatty acid synthase (FAS), acetyl CoA carboxylase 1 (ACC1) and sterol regulatory elementary binding protein-1 (SREBP-1). Results: Differentiation of AFMSCs into adipocytes was found by oil-red O staining. Zoledronate influenced the differentiation of AFMSCs into adipocytes in a dose- and time-dependent manner. At 7 days of culture, the expressions of FAS and showed no significant differences compared to that of the control regardless of the dose of zoledronate. Very little ACC1 expression was found. However, the expressions of these three markers were remarkably increased at 14 days of culture. Of them, the ACC1 expression was significantly increased by 10−8 M and 10−6 M of zoledronate. At 21 days of culture, there were no effects of zoledronate on the expressions of FAS and SREBP-1. However, the ACC1 expression was decreased with an increasing dose of zoledronate (P<.05). Conclusion: This study shows that AFMSCs can be differentiated into adipocytes. The induction of this differentiation following zoledronate treatment appears to be dose dependent and time-of-culture dependent. (Korean J Blood Transfus 2008;19:231-238)
Keywords : Amniotic fluid, Mesenchymal stem cell, Adipocyte, Zoledronate


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