Results of RhD testing by the tube and plate methods using a serially diluted anti-D reagent
| Dilution ratio | Reactivity | Time to agglutination | ||
|---|---|---|---|---|
| Tube | Plate | Plate | ||
| 1X | 4＋ | 3＋ | 10 s | |
| 2X | 4＋ | 3＋ | 10 s | |
| 4X | 4＋ | 3＋ | 10 s | |
| 8X | 4＋ | 3＋ | 10 s | |
| 16X | 4＋ | 3＋ | 13 s | |
| 32X | 4＋ | 2＋ | 17 s | |
| 64X | 4＋ | 2＋ | 26 s | |
| 128X | 4＋ | 2＋ | 30 s | |
| 256X | 3＋ | 2＋ | 45 s | |
These are representative data from at least three experiments. A dilution ratio of 1 indicates that the original anti-D reagent was undiluted.