Fig. 2. Analysis of platelet antibody testing by flow cytometry. (A) The initial platelet gate, P1, was established around platelets on the FSC/SSC plot. (B) The second platelet gate, P2, was defined to encompass platelets on the FSC/CD41a PE plot. (C) Histogram of IgG for patient and control was presented. MFI values were derived from the peaks observed on the IgG FITC histogram of the gated platelets. (D) Histogram of IgM for patient and control was presented. MFI values were derived from the peaks observed on the IgM BV605 histogram of the gated platelets.
Abbreviations: FSC, forward scatter; SSC, side scatter; PE, phycoerythrin; MFI, mean fluorescence intensity; FITC, fluorescein isothiocyanate; BV605, brilliant violet 605.
© 2023 Korean J Blood Transfus
