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Ex vivo Expansion of Human Natural Killer Cells from Blood Retained in a Disposable Platelet Apheresis Set
혈소판성분채집세트내 잔여혈액에서 자연살생세포의 체외 증폭
The Korean Society of Blood Transfusion 2012;23:28−37
Published online April 30, 2012
© 2012 The Korean Society of Blood Transfusion.

Dong-Jun Shin1, Duck Cho1,2, Youn-Young Jang1, Ji-Yun Park1,3, Ju-Sun Kim1,3, Sang-Ki Kim1,3, Je-Jung Lee1, Min-Joong Jang2, Myung-Geun Shin2, Dong-Wook Ryang2
신동준1ㆍ조덕1,2ㆍ장윤영1ㆍ박지윤1,3ㆍ김주선1,3ㆍ김상기1,3ㆍ이제중1ㆍ장민중2ㆍ신명근2ㆍ양동욱2
Abstract
Background:Natural killer cells expanded from human peripheral blood (PB) have been used in cancer immunotherapy research. Although most research teams have access to human PB, it is necessary to find a source of blood that can be easily obtained. We have tested the possibility of using blood retained in a disposable platelet apheresis set as an alternative source, with special interest in expansion of NK cells for use in cancer immunotherapy research.
Methods:For expansion of NK cells, peripheral blood mononuclear cells (PBMCs) were isolated from an MCS+ platelet apheresis kit (Haemonetics, Braintree, USA) and PB from the same donor (n=7) and co-cultured with 100-Gy gamma ray-irradiated K562 cells expressing the 4-1BB ligand and membrane-bound IL-15 for three weeks in RPMI1640 medium in the presence of IL-2 and IL-15. Cytotoxicity was measured using WST-1 at 1:1, 2:1, and 4:1 effector-to-target (E:T) ratios for a period of four hours.
Results:Mean rate of expansion of NK cells was 1,097-fold and their purity was 94.4% from blood retained in a disposable platelet apheresis set; mean rate of expansion of NK cells was 953-fold and their purity was 92.0% from PB after a period of three weeks. No differences in cytotoxicity against K562, 697, Raji, and RPMI8226 were observed between NK cells expanded from two blood sources.
Conclusion:Blood retained in a disposable platelet apheresis set is a useful and convenient source for expansion of NK cells for use in cancer immunotherapy research. (Korean J Blood Transfus 2012;23: 0-37)
Keywords : Natural killer cell, Ex vivo expansion, Apheresis set

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