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Development of HLA-A, -B and -DR Typing Method Using Next-Generation Sequencing
차세대염기서열분석법을 이용한 HLA-A, -B 그리고 -DR 형별 분석법 개발
Korean J Blood Transfus 2018;29:310−319
Published online December 31, 2018;
© 2018 The Korean Society of Blood Transfusion.

Dong Hee Seo, Jeong Min Lee, Mi Ok Park, Hyun Ju Lee, Seo Yoon Moon, Mijin Oh, So Young Kim, Sang-Heon Lee, Ki-Eun Hyeong, Hae-Jin Hu, Dae-Yeon Cho

LabGenomics Clinical Laboratories, Seongnam, Korea
Dong Hee Seo
LabGenomics Clinical Laboratories, 700 Daewangpangyo-ro, Bundang-gu, Seongnam 13488, Korea
Tel: 82-31-628-0730, Fax: 82-31-628-0701, E-mail:, ORCID:
Received April 23, 2018; Revised September 17, 2018; Accepted November 7, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background: Research on next-generation sequencing (NGS)-based HLA typing is active. To resolve the phase ambiguity and long turn-around-time of conventional high resolution HLA typing, this study developed a NGS-based high resolution HLA typing method that can handle large-scale samples within an efficient testing time.
Methods: For HLA NGS, the condition of nucleic acid extraction, library construction, PCR mechanism, and HLA typing with bioinformatics were developed. To confirm the accuracy of the NGS-based HLA typing method, the results of 192 samples HLA typed by SSOP and 28 samples typed by SBT compared to NGS-based HLA-A, -B and -DR typing.
Results: DNA library construction through two-step PCR, NGS sequencing with MiSeq (Illumina Inc., San Diego, USA), and the data analysis platform were established. NGS-based HLA typing results were compatible with known HLA types from 220 blood samples.
Conclusion: The NSG-based HLA typing method could handle large volume samples with high-throughput. Therefore, it would be useful for HLA typing of bone marrow donation volunteers.
Keywords : HLA, NGS, HLA typing


December 2018, 29 (3)
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