search for




 

Development of HLA-A, -B and -DR Typing Method Using Next-Generation Sequencing
차세대염기서열분석법을 이용한 HLA-A, -B 그리고 -DR 형별 분석법 개발
Korean J Blood Transfus 2018;29:310−319
Published online December 31, 2018;  https://doi.org/10.17945/kjbt.2018.29.3.310
© 2018 The Korean Society of Blood Transfusion.

Dong Hee Seo, Jeong Min Lee, Mi Ok Park, Hyun Ju Lee, Seo Yoon Moon, Mijin Oh, So Young Kim, Sang-Heon Lee, Ki-Eun Hyeong, Hae-Jin Hu, Dae-Yeon Cho
서동희ㆍ이정민ㆍ박미옥ㆍ이현주ㆍ문서윤ㆍ오미진ㆍ김소영ㆍ이상헌ㆍ형기은ㆍ허혜진ㆍ조대연

LabGenomics Clinical Laboratories, Seongnam, Korea
랩지노믹스의학연구소
Dong Hee Seo
LabGenomics Clinical Laboratories, 700 Daewangpangyo-ro, Bundang-gu, Seongnam 13488, Korea
Tel: 82-31-628-0730, Fax: 82-31-628-0701, E-mail: seo2023@nate.com, ORCID: http://orcid.org/0000-0003-0828-3773
Received April 23, 2018; Revised September 17, 2018; Accepted November 7, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Research on next-generation sequencing (NGS)-based HLA typing is active. To resolve the phase ambiguity and long turn-around-time of conventional high resolution HLA typing, this study developed a NGS-based high resolution HLA typing method that can handle large-scale samples within an efficient testing time.
Methods: For HLA NGS, the condition of nucleic acid extraction, library construction, PCR mechanism, and HLA typing with bioinformatics were developed. To confirm the accuracy of the NGS-based HLA typing method, the results of 192 samples HLA typed by SSOP and 28 samples typed by SBT compared to NGS-based HLA-A, -B and -DR typing.
Results: DNA library construction through two-step PCR, NGS sequencing with MiSeq (Illumina Inc., San Diego, USA), and the data analysis platform were established. NGS-based HLA typing results were compatible with known HLA types from 220 blood samples.
Conclusion: The NSG-based HLA typing method could handle large volume samples with high-throughput. Therefore, it would be useful for HLA typing of bone marrow donation volunteers.
Keywords : HLA, NGS, HLA typing

 

December 2018, 29 (3)
Full Text(PDF) Free

Social Network Service

Cited By Articles
  • CrossRef (0)

Author ORCID Information
Services