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Freezing and Washing of Red Blood Cells Using Haemonetics ACP 215
Haemonetics ACP 215를 이용한 적혈구의 동결과 세척
Korean J Blood Transfus 2018;29:291−300
Published online December 31, 2018;
© 2018 The Korean Society of Blood Transfusion.

Kyoung Won Youn1, Kyoung Young Choi2, Sun Ah Lee3, Hyuk Ki Min1, Jaehyun Kim1

Blood Transfusion Research Institute, Korean Red Cross1, Wonju, Jungbu Blood Laboratory Center, Korean Red Cross2, Daejeon, Department of Laboratory Medicine, Myongji St. Mary's Hospital3, Seoul, Korea
대한적십자사 혈액수혈연구원1, 대한적십자사 중부혈액검사센터2, 명지성모병원 진단검사의학과3
Jaehyun Kim
Blood Transfusion Research Institute, Korean Red Cross, 50 Hyeoksin-ro, Wonju 26465, Korea
Tel: 82-33-811-0221, Fax: 82-33-811-0240, E-mail:, ORCID:
Received September 7, 2018; Revised October 16, 2018; Accepted October 30, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background: The use of a functionally closed system for the glycerolization and deglycerolization of red blood cells (RBCs) allows for prolonged post-thaw storage for more than 24 hours. The aim of this study was to assess glycerolization and deglycerolization processing for RBCs using a high glycerol method in the automated, closed system provided by Haemonetics ACP 215.
Methods: Thirty-five packed RBCs were glycerolized using the ACP 215 to a final concentration of 40% (wt/vol). The units were either frozen as such (n=30) or excess glycerol was removed (n=5) before freezing. After storage at –80°C, the units were thawed, deglycerolized and resuspended in SAG-M. The frozen-thawed RBCs were stored at 4°C, and analyzed for their stability and in vitro quality.
Results: No prefreeze excess glycerol removal units showed significantly less potassium leakage during post-thaw storage compared to the prefreeze excess glycerol removal units. All measurements of the stability and in vitro quality of thawed RBCs prepared from frozen RBCs without the prefreeze removal of excess glycerol during post-thaw storage at 4°C for 7 days were acceptable to the American Blood Bank Association’s standards and European standards.
Conclusion: RBCs frozen without prefreeze removal of excess glycerol and the ACP 215 simplifies cryopreservation procedure and increases the stability of frozen-thawed RBCs. This increases the practical applicability of cryopreserved RBCs in blood transfusion practice.
Keywords : Red blood cells, Cryopreservation, Glycerolization, Deglycerolization


December 2018, 29 (3)
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