The Korean Journal of Blood Transfusion : eISSN 2383-6881 / pISSN 1226-9336

Fig. 2.

Download original image
Fig. 2.

HLA NGS workflow. HLA NGS workflow started with multiplex PCR (PCR I) of HLA-A, -B, and -DRB1. After PCR I, the amplicons were diluted, pooled and bar-coded (PCR II). The bar-coded libraries were pooled in equimolar concentrations and bead-purified. The resulting pooled library was denatured, diluted, and sequenced on MiSeq/MiSeqDx instruments using the paired-end 2×250 cycle MiSeq Reagent Kit. The sequence data were exported and analyzed using HLA NGS analysis software (PEERE) with 3.19.0 IMGT/HLA database serving as the reference.

Korean J Blood Transfus 2018;29:310-9
© 2018 Korean J Blood Transfus