The Korean Journal of Blood Transfusion : eISSN 2383-6881 / pISSN 1226-9336

Table. 1.

Table. 1.

Effect of long-term storage at –70°C on the quantitation of HBV DNA, HCV RNA and HIV-1 RNA national reference standard for in vitro diagnostics

National Reference Standard Mean log10 viral loads (95% CI) at: P value

00 MO 12 MO 15 MO 24 MO 36 MO 48 MO 60 MO 72 MO
HBV DNA Working Standard (IVD-12/019)
 Panel 1 6.55 (6.46∼6.63) 6.50 (6.43∼6.57) 6.51 (6.43∼6.59) 6.47 (6.39∼6.56) 6.56 (6.43∼6.70) 6.51 (6.41∼6.61) 6.47 (6.45∼6.48) 6.47 (6.47∼6.48) 0.88
 Panel 2 4.85 (4.74∼4.93) 4.85 (4.71∼4.98) 4.75 (4.68∼4.82) 4.76 (4.70∼4.81) 4.89 (4.79∼5.00) 4.80 (4.73∼4.88) 4.83 (4.77∼4.89) 4.83 (4.79∼4.87) 0.62
 Panel 3 4.23 (4.16∼4.29) 4.23 (4.13∼4.32) 4.24 (4.17∼4.30) 4.22 (4.21∼4.23) 4.29 (4.20∼4.37) 4.21 (4.12∼4.30) 4.28 (4.25∼4.31) 4.29 (4.27∼4.31) 0.59
 Panel 4 3.61 (3.54∼3.67) 3.61 (3.61∼3.62) 3.64 (3.49∼3.76) 3.64 (3.53∼3.74) 3.61 (3.56∼3.66) 3.53 (3.28∼3.78) 3.68 (3.62∼3.74) 3.69 (3.58∼3.78) 0.48
 Panel 5 2.94 (2.79∼3.05) 3.08 (3.05∼3.11) 3.04 (3.01∼3.08) 2.99 (2.94∼3.04) 2.93 (2.85∼3.00) 3.01 (2.94∼3.08) 3.10 (3.06∼3.15) 2.98 (2.91∼3.03) 0.83
HBV Recombinant DNA (IVD-12/020) 6.05 (5.99∼6.09) 5.90 (5.83∼5.97) 5.73 (5.64∼5.82) 5.65 (5.56∼5.75) 5.90 (5.82∼5.99) 7.02 (6.91∼7.13) 6.92 (6.06∼7.41) 7.01 (6.68∼7.20) 0.50
HCV RNA Working Standard (IVD-12/021)
 Panel 1 5.23 (5.13∼5.32) 5.33 (5.31∼5.35) 5.28 (5.23∼5.31) 5.13 (5.12∼5.13) 5.31 (5.29∼5.33) 5.22 (5.17∼5.26) 5.21 (5.19∼5.23) 5.13 (5.07∼5.19) 0.42
 Panel 2 3.34 (3.26∼3.40) 3.39 (3.36∼3.43) 3.43 (3.37∼3.49) 3.27 (3.26∼3.29) 3.56 (3.53∼3.58) 3.55 (3.53∼3.57) 3.44 (3.43∼3.45) 3.42 (3.41∼3.43) 0.73
 Panel 3 2.80 (2.70∼2.88) 2.85 (2.77∼2.93) 2.88 (2.75∼3.01) 2.67 (2.64∼2.71) 2.92 (2.90∼2.93) 3.04 (2.99∼3.09) 2.90 (2.81∼2.99) 2.89 (2.83∼2.95) 0.63
 Panel 4 2.20 (2.06∼2.31) 2.39 (2.16∼2.56) 2.31 (2.24∼2.37) 2.01 (1.95∼2.07) 2.41 (2.27∼2.55) 2.49 (2.30∼2.67) 2.30 (2.19∼2.41) 2.34 (2.18∼2.46) 0.66
HCV Recombinant DNA (IVD-12/022) 7.44 (7.21∼7.59) 7.70 (7.65∼7.74) 7.79 (7.73∼7.86) 7.06 (6.93∼7.18) 7.50 (7.46∼7.55) >8.00 (N/A) >8.00 (N/A) >8.00 (N/A) 0.67
HIV-1 RNA Working Standard (IVD-12/007)
 Panel 1 4.32 (4.26∼4.37) 4.19 (4.17∼4.21) 4.25 (4.22∼4.27) 3.76 (3.74∼3.77) 4.22 (4.18∼4.27) 4.23 (4.19∼4.28) 4.22 (4.20∼4.23) 4.16 (4.10∼4.21) 0.74
 Panel 2 2.36 (2.22∼2.47) 2.26 (2.22∼2.29) 2.14 (2.08∼2.19) 1.82 (1.72∼1.92) 2.17 (2.02∼2.32) 2.13 (1.90∼2.37) 2.30 (2.16∼2.44) 2.25 (2.19∼2.31) 0.82
 Panel 3 2.10 (2.08∼2.12) 1.45 (1.34∼1.57) 1.65 (1.29∼2.00) 1.35 (1.25∼1.46) 1.54 (1.28∼1.80) 1.54 (1.28∼1.79) 1.56 (1.31∼1.81) 1.79 (1.76∼1.82) 0.98
 Panel 4 1.45 (1.35∼1.53) 1.33 (1.27∼1.39) <1.30 (N/A) <1.30 (N/A) <1.30 (N/A) 1.46 (1.28-1.64) <1.30 (N/A) <1.30 (N/A) 0.93
HIV-1 Recombinant DNA (IVD-12/008) >7.00 (N/A) >7.00 (N/A) >7.00 (N/A) >7.00 (N/A) >7.00 (N/A) >7.00 (N/A) >7.00 (N/A) >7.00 (N/A) N/A

Abbreviations: MO, month; CI, confidence interval; N/A: not applicable.

Korean J Blood Transfus 2018;29:171-81 https://doi.org/10.17945/kjbt.2018.29.2.171
© 2018 Korean J Blood Transfus